Mdm2 CLIA Kit for Mouse(Mus musculus) | EIAab.comMdm2 CLIA Kit for Mouse(Mus musculus)

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Mdm2 (Gene name),

E3 ubiquitin-protein ligase Mdm2 (Protein name ), MDM2_MOUSE from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Mdm2;

Protein name:

E3 ubiquitin-protein ligase Mdm2;

Alternative:

Oncoprotein Mdm2;Double minute 2 protein;p53-binding protein Mdm2;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Binds p53/TP53, TP73/p73, ARF/P14, PML, RBL5 and RP11, and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53/TP53 and WWOX. Interacts with CDKN2AIP, RFWD3, USP7, PYHIN1, UBXN6, and RBBP6. Isoform Mdm2-F does not interact with p53/TP53. Interacts with AARB1. Interacts (isoform 2) with PSMA3. Found in a trimeric complex with MDM2, MDM4 and UPB2. Interacts with USP2 (via N-terminus and C-terminus). Interacts with MDM4. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Part of a complex with DAXX, MDM2 and USP7. Interacts directly with DAXX and USP7. Interacts (via C-terminus) with RASSF1 isoform A (via N-terminus); the interaction is independent of TP53. Interacts with DAXX. Interacts with APEX1; the interaction leads to its ubiquitination and degradation. Interacts with RYBP; this inhibits ubiquitination of TP53. Identified in a complex with RYBP and p53/TP53. Also component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in regulating p53/TP53 stabilization and activity. Binds directly both p53/TP53 and TRIM28. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor responses with DNA damage. Interacts directly with both MDM2 and ERBB4 in the complex (By similarity). Interacts with AARB2, MTBP and TBRG1.

Function:

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.

Subcellular Location:

Nucleus nucleoplasm Cytoplasm Nucleus nucleolus Colocalizes with RASSF1 isoform A in the nucleus (By similarity). Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

Protein Attributes:

Sequence length:

489

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

489:

GKTGHLMSCF | TCAKKLKKRN | KPCPVCRQPI | QMIVLTYFN

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Mm.22670

SMR:

P23804

String:

P23804

KEGG:

mmu:17246

Pfam:

PF02201

Uniprot:

P23804

FOR

ELISA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

E12872m

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MSDS:

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Packing:

96T

ELISA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

E12872h

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MSDS:

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Packing:

96T

ELISA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

E12872d

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Packing:

96T

CLIA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

U12872m

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

U12872h

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

U12872d

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

P12872Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse E3 ubiquitin-protein ligase Mdm2

Cat.:

P12872Rb-m

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse E3 ubiquitin-protein ligase Mdm2

Protein for Mouse E3 ubiquitin-protein ligase Mdm2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"Tumorigenic potential associated with enhanced expression of a gene that is amplified in a mouse tumor cell line."
Fakharzadeh S.S. , Trusko S.P. , George D.L.
EMBO J.10:1565-1569(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MDM2-P90)
2.

"Genomic organization of the mouse double minute 2 gene."
Jones S.N. , Ansari-Lari M.A. , Hancock A.R. , Jones W.J. , Gibbs R.A. , Donehower L.A. , Bradley A.
Gene175:209-213(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM MDM2-P90)
strain: 129/Sv.
3.

"The organization and expression of the mdm2 gene."
de Oca Luna R.M. , Tabor A.D. , Eberspaecher H. , Hulboy D.L. , Worth L.L. , Colman M.S. , Finlay C.A. , Lozano G.
Genomics33:352-357(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM MDM2-P90)
strain: 129/Sv.
4.

"Multiple murine double minute gene 2 (MDM2) proteins are induced by ultraviolet light."
Saucedo L.J. , Myers C.D. , Perry M.E.
J. Biol. Chem.274:8161-8168(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS MDM2-P90 AND MDM2-P76);TISSUE SPECIFICITY;INDUCTION
5.

"The transcriptional landscape of the mammalian genome."
Carninci P. , Kasukawa T. , Katayama S. , Gough J. , Frith M.C. , Maeda N. , Oyama R. , Ravasi T. , Lenhard B. , Wells C. , Kodzius R. , Shimokawa K. , Bajic V.B. , Brenner S.E. , Batalov S. , Forrest A.R. , Zavolan M. , Davis M.J. , more...
Science309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6J.
strain: NOD.
tissue: Lung.
tissue: Thymus.
6.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
strain: C57BL/6.
7.

"Cooperative signals governing ARF-mdm2 interaction and nucleolar localization of the complex."
Weber J.D. , Kuo M.-L. , Bothner B. , DiGiammarino E.L. , Kriwacki R.W. , Roussel M.F. , Sherr C.J.
Mol. Cell. Biol.20:2517-2528(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOLAR LOCALIZATION SIGNAL
8.

"Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage."
Khosravi R. , Maya R. , Gottlieb T. , Oren M. , Shiloh Y. , Shkedy D.
Proc. Natl. Acad. Sci. U.S.A.96:14973-14977(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION BY ATM
9.

"A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2."
Boyd M.T. , Vlatkovic N. , Haines D.S.
J. Biol. Chem.275:31883-31890(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH MTBP
10.

"Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin."
Shenoy S.K. , McDonald P.H. , Kohout T.A. , Lefkowitz R.J.
Science294:1307-1313(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH AARB2
11.

"PML regulates p53 stability by sequestering Mdm2 to the nucleolus."
Bernardi R. , Scaglioni P.P. , Bergmann S. , Horn H.F. , Vousden K.H. , Pandolfi P.P.
Nat. Cell Biol.6:665-672(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH PML AND RPL11;SUBCELLULAR LOCATION
12.

"A novel nuclear interactor of ARF and MDM2 (NIAM) that maintains chromosomal stability."
Tompkins V.S. , Hagen J. , Frazier A.A. , Lushnikova T. , Fitzgerald M.P. , di Tommaso A.D. , Ladeveze V. , Domann F.E. , Eischen C.M. , Quelle D.E.
J. Biol. Chem.282:1322-1333(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TBRG1

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